A randomized double-blind clinical trial of treating NAFLD demonstrated that licorice, the powder from the root of GL, supplementation contributes to a reduction of ALT levels and liver steatosis in patients with lifestyle modification, suggesting that licorice supplementation can improve the effectiveness of lifestyle modification alone in treating NAFLD (Rostamizadeh et al., 2022). This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.