STX2 and hemolytic-uremic syndrome: Importantly, strain EC727A3 contains the virulence genes stx2A and stx2B that produce Shiga toxin, which may cause self-limiting diarrhoeal disease and sometimes bloody diarrhea as well as complications such as hemorrhagic colitis and hemolytic uremic syndrome (HUS) (Fitzpatrick, 1999; Launders et al., 2016; Mcfarland et al., 2017).