The OPLS-DA model revealed that several ECM-associated genes including COL8A1, COL8A2, COL4A2, COL27A1, COLEC12, COL10A1, PCOLCE, COLEC11, COL6A2, COL6A3, COL13A, COL5A2, MMP14, COL5A1, COL4A1, COL1A2, COL1A1, and COL6A1 were significantly overexpressed in ameloblastoma compared to corresponding normal oral tissue with the criteria of FC >2 and P value <0.01. This evidence concerns the gene COL4A1 and ameloblastoma.