CD4 and neoplasm: In this study, we examined the immune infiltration in the circulatory system and TME of homograft mice treated with tumor vaccine using flow cytometry and ELISA, and found that killer immune cells such as CD8, CD4, and NK were significantly increased after treatment, while immunosuppressive cells MDSC and immunosuppressive cells with PD-1 and LAG3 antigens were increased after PD-1treatment and significantly decreased after vaccine combination therapy.