In this study we used a novel rodent hepacivirus mouse model to characterize hepatic iNKT cell subsets during different stages of an HCV-related virus infection in vivo. We report the activation of a highly diverse iNKT cell response that was associated with an activation of iNKT cells exhibiting a mixed NKT1/NKT2 signature and production of the type 2 cytokines IL-4 and IL-13. This evidence concerns the gene IL13 and viral infectious disease.