TNF and neoplasm: Tumor slices were incubated with 1 nM LY6G6D/CD3 TcE or TNP/CD3 TcE, a control TcE that binds to the irrelevant antigen trinitrophenol, for 48 hours and activation of TILs was assessed by quantifying cytokines in the cell culture supernatant and by staining the tumor slices with Granzyme B. Treatment with LY6G6D TcE induced more than 1.5-fold secretion of IFN-γ, IP-10 (5/5 LY6G6D positive samples), and Granzyme B, IL-2, TNFα and MCP-1 (4/5 LY6G6D positive samples) (Figure 3B).