Among the 47 functional human Vβ genes, the usages of TRBV2, TRBV4-1, TRBV4-2, TRBV5-4, TRBV6-4, TRBV6-8, TRBV7-3, TRBV7-4, TRBV7-6, TRBV7-7, TRBV10-1, TRBV10-3, TRBV12-3, TRBV14, TRBV18, TRBV20-1, TRBV24-1, TRBV25-1, and TRBV30 in healthy donors are significantly higher than in cancer patients; the usages of TRBV11-2 and TRBV16 in cancer patients are significantly higher than in healthy donors (Figure 1A, p < 0.05, Mann-Whitney U test). This evidence concerns the gene TRBV16 and cancer.