From a holistic perspective, functional enrichment analysis found that FA-metabolism associated genes upregulated in Macro1 were significantly enriched in leukotriene (ALOX5/ALOX15B/DPEP1/PTGR1) and prostaglandin (PLA2G4A/PLA2G5/PTGS1/PTGS2/PTGR1) synthesis, while in Macro3, only PTGDS, PLA2G6, and PNPLA3 were involved in the eicosanoid metabolism (Figure 2G), suggesting that genes involved in leukotriene synthesis were remarkably altered between low- and high-grade glioma. Here, PNPLA3 is linked to glioma.