When using cryo-thermal therapy to treat mice implanted with B16F10 melanoma, a strong systemic and melanoma-specific antitumor immune memory was generated and CD4+ T cell depletion abrogated the antitumor memory response in some mice, while CD8+ T cell depletion demonstrated no inhibition of antitumor memory compared to non-depleted controls (62). This evidence concerns the gene CD4 and melanoma.