To verify the results of RNA sequencing, four genes related to the tumor were further assessed by qPCR RASAL2 from Ras signaling pathway, UGT1A4 from chemical carcinogenesis, PDE3A from cAMP signaling pathway, SerpinB5 from microRNAs in cancer, and p53 signaling pathway. The gene discussed is PDE3A; the disease is neoplasm.