SMURF2 and hepatocellular carcinoma: Moreover, except SNU475 cell, the expression of the markers of TGF-β1 signaling, including Smad7, Smurf2, TGF-β1, and p-Smad2, was highly expressed in HCC cell lines compared with those in THLE3 cells (Figures 3(b) and 3(c)).