For BLCA, the combined diagnosis of HSP90α+CEA+AFP has the largest AUC, but there is no significant difference between HSP90α+CEA and HSP90α+CEA+AFP in sensitivity and specificity, the both AUC are 1, while the sensitivity and specificity of both groups are 100%. The gene discussed is HSP90AA1; the disease is bladder transitional cell carcinoma.