The more recent Denmark ADDITION-PRO study, which was one of the largest longitudinal risk-stratified cohort study, demonstrated that total GLP-1 responses, especially GLP-1 responses at 120 min during OGTT, were reduced by 16%–21% in pre-DM and T2DM compared to subjects with NGT (24). This evidence concerns the gene GLP1R and type 2 diabetes mellitus.