To date, a number of ctDNA biomarkers have been identified and used for the diagnosis and prognosis of BC, including tumor protein p53 (TP53), AKT1, and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) etc., but they still lack specificity for TNBC [9]. This evidence concerns the gene PIK3CA and breast cancer.