At last, we used qRT-PCR to detect the expression of seven pyroptosis-related genes in sarcoma cells (RH30 and SW982) and human skeletal muscle cells, compared with human skeletal muscle cells, PODXL2, LRRC17, GABRA3, SCUBE3 and RFLNB genes show high expression levels in tumor cells, while IGHG2 and HLF show low expression levels in tumor cells. This evidence concerns the gene RFLNB and sarcoma.