Remarkably, deletion of both tie1 and tie2 in a Δvprh/Δhns1 background (Δvprh/Δhns1/Δtie1/Δtie2) completely abolished the cell death and IL-1β secretion observed upon infection of BMDMs with the Δvprh/Δhns1 strain (Figure 7—figure supplement 1a,b), without affecting TNFα secretion (Figure 7—figure supplement 1b). The gene discussed is TIE1; the disease is infection.