Using real-time microscopy, we observed that addition of either inhibitors, MCC950 (blocks ASC oligomerization by inhibiting the canonical and non-canonical NLRP3 inflammasome Shao et al., 2015) or Vx765 (a potent and selective competitive inhibitor of Caspase-1 Church et al., 2008) resulted in delayed and reduced cell death during infection with Δvprh/Δhns1 bacteria (~40% reduction as measured by calculating the AUC), compared with a dimethyl sulfoxide (DMSO)-treated control (Figure 2a and b). This evidence concerns the gene CASP1 and infection.