Additional hallmark pyroptotic processes, such as the cleavage and release of Caspase-1 and GSDMD, were apparent upon infection of BMDMs with the Δvprh/Δhns1 strain; however, they were undetected upon inactivation of T6SS3 or upon the addition of inflammasome inhibitors (Figure 2d and e). This evidence concerns the gene GSDMD and infection.