MLKL and infection: Approximately 3.5×104 wild-type (B6J), Nlrp1-/-, Nlrp3-/-, and Mlkl-/- bone marrow-derived macrophages (BMDMs) were seeded into 96-well plates in six replicates and were primed using lipopolysaccharides (LPS) (100 ng/mL) for 3 hr prior to infection with V. proteolyticus strains at multiplicity of infection (MOI) 5.