Taking 0.54 as the optimal cutoff point according to the Youden index in ROC tests, patients were assigned to the LFR low (< 0.54) or high (≥ 0.54) subgroups, and patients in the LFR low group were more likely to be characterized by criteria such as T3 + T4 (P < 0.01), stage 3 (P < 0.01), tumor deposits (P = 0.01), high CEA (P < 0.01), or CA19-9 levels (P = 0.04; Table 1). The gene discussed is CEACAM5; the disease is neoplasm.