We then treated cells with siMELK, which resulted in downregulation of FOXM1, AXIN2, GSK-3β, and β-catenin, suggesting that MELK is the upstream regulator of these molecules in the Wnt/β-catenin signaling pathway in ACC (Supplementary Fig. 6B). The gene discussed is GSK3B; the disease is adrenal cortex carcinoma.