We then treated cells with siMELK, which resulted in downregulation of FOXM1, AXIN2, GSK-3β, and β-catenin, suggesting that MELK is the upstream regulator of these molecules in the Wnt/β-catenin signaling pathway in ACC (Supplementary Fig. 6B). Here, FOXM1 is linked to adrenal cortex carcinoma.