Given the critical role of cytokines in cell–cell interaction, we applied RT-qPCR-based cytokines array analysis, combining with ELISA, to screen the changes of transcription level of cytokines in three macrophages (M0, MS and MR) and identified CXCL5 as the rational target that accountable for TAMs-induced chemoresistance in gastric cancer. This evidence concerns the gene CXCL5 and gastric cancer.