Annexin V‐FITC/PI flow cytometry assays showed that doxorubicin had a strong effect on the induction of apoptosis in both breast cancer cell types (MCF‐7 and MDA‐MB‐231); the rate of apoptosis increased significantly with increasing drug dose and time (p < 0.01) (Figure 3D). The gene discussed is ANXA5; the disease is breast carcinoma.