Proteomic analysis of the Kveim reagent, compared with control spleen tissue, revealed the presence of three proteins detected only in sarcoidosis tissue: vimentin, tubulin, and α-actinin-4; the capacity of these proteins to induce a pro-inflammatory response in peripheral blood mononuclear cells (PBMCs) of patients with sarcoidosis, patients with tuberculosis, and healthy controls were tested. This evidence concerns the gene VIM and tuberculosis.