ROI analyses of SYNGR3+ cell compartmentalization within the tumor microenvironment were performed by defining the tumor as 25 μm on either side of the border of the tumor core and revealed that SYNGR3+/CD3+ and SYNGR3+/CD45+ cells were primarily confined to stromal regions (pan-CK and p16 negative) compared with the cancer cell islet (pan-CK and p16 positive) regions (Fig. 5D; Supplementary Fig. S3E in Supplementary Materials and Methods S1). This evidence concerns the gene SYNGR3 and neoplasm.