We thus further examined the EMT markers (E-cadherin, N-cadherin, vimentin, and snail) by Western blot to investigate whether ARPC5 could affect EMT in HCC cells, the results showed that knockdown of ARPC5 reduced the expression of N-cadherin, Vimentin, and Snail whereas increased E-cadherin expression in HCC-LM3 and MHCC 97-H cells (Figure 12G). The gene discussed is ARPC5; the disease is hepatocellular carcinoma.