TWIST1 and infection: PCR analysis showed that lentiviral vector knockdown of MYOF in NCI-N87 and MKN45 cells suppressed the expression of Twist1 and Arpc3 mRNAs, which encode markers of cell motility (P<0.0001 each), whereas infection with vectors overexpressing MYOF enhanced the expression of Twist1 and Arpc3 mRNAs (P<0.001 each) (Figures 4D, E; Supplementary Figures 1C, D).