Given that the COG risk classification already incorporates age, MYCN amplification status, and INSS stage factors, we created a nomogram (Figure 4E) incorporating only age and the FRG signature risk score to further investigate the role of age in predicting the OS in NB patients in the validation cohort, as age showed significant differences in univariate and multivariate Cox regression analyses. The gene discussed is MYCN; the disease is neuroblastoma.