MYH14 and familial dilated cardiomyopathy: Myosin mutants from HCM (R403Q, R453C) and DCM (S532P) were compared using the load-clamp laser trap assay, and it was found that maximal force-generating capacity increased 50% in R403Q-myosin and 80% in R453C-myosin and decreased 65% in S532P-myosin (Debold et al., 2007; Sommese et al., 2013).