We used the integrated data (integrated four datasets) to validate our hypothesis, and the investigated results revealed that the ROC curve of the expression levels of these top ten hub genes (RPS27A, MAPK1, STAT1, TLR4, RPS6, FBL, TLR2, RPS3, RPS2, and GART) showed excellent diagnostic value for peripheral blood with PAH and controlled peripheral blood cells (Figure 9A). The gene discussed is TLR4; the disease is pulmonary arterial hypertension.