Interestingly, the overlap between mRNAs up-regulated in Mir34a-deficient adenomas and tumoroids, though limited, was statistically highly significant, and among the 23 mRNAs significantly up-regulated in both Mir34a-deficient adenomas and tumoroids were three factors involved in Wnt signaling (Dkk2, Fzd10 and Wnt10a) (Fig. 6E), suggesting that the tumor cell-autonomous repression of Wnt signaling by miR-34a may be a critical mechanism of miR-34a mediated tumor suppression, as reported previously 51, 52. This evidence concerns the gene FZD10 and adenoma.