From results in Fig. 6, we could summarize that the abilities of stemness, proliferation, migration and invasion in HCC cells with SPC25 overexpression were significantly suppressed by AZD-7648, MK-2202 and the silence of Notch1, which respectively inhibited the activation of DNA-PK, AKT and Notch. The gene discussed is NOTCH1; the disease is hepatocellular carcinoma.