To further verify that SOX2 and NANOG were the main downstream regulated genes of the DNA-PK/AKT/Notch1 signaling pathway that promoted the stemness of HCC cells with upregulated SPC25 expression, we first used the PROMO website to predicate putative RBP-Jκ binding sites in the SOX2 and NANOG promoter region and identified an RBP-Jκ binding site in the -2007 to -1996 region of SOX2 and in the -2213 to -2202 region of NANOG. The gene discussed is PRKDC; the disease is hepatocellular carcinoma.