To explore the correlation between TRPM4 and the metastasis of CRC in vivo, we next used two experimental metastasis mouse models to investigate the effects of TRPM4 on metastasis: (1) implantation of cells into the abdominal cavity of female nude mice by intraperitoneal injection; (2) injection of cells into the spleen of female nude mice. The gene discussed is TRPM4; the disease is colorectal carcinoma.