Here, we observed that CD163-3D4/21 cells were able to support the infections of all tested PRRSV2 strains, including NADC30-like PRRSV (SD17-38) and NADC34-like PRRSV (Anheal-1), in spite of their failure to infect MARC-145 cells, which is the first report as far as we know. Here, CD163 is linked to infection.