CD8A and primary biliary cholangitis: HBRV has been detected in tissues and by immune response in PBC patients○Biliary epithelium: Proviral integrations (58%), electron microscopy, RT-PCR○Lymph nodes: Proviral integrations (45%), RT-PCR, immunochemistry (75%)○Liver: Proviral integrations (13%), Nested PCR (12%), RT-PCR (20%)○Immune responses: Flow cytometry CD8+ PBMC (43%), interferon-γ release assay in PBMC (50%) and intrahepatic lymphocytes (100%), IgG reactivity to HBRV Su (11%)