PRNP and prion disease: Similar to naturally occurring prions, recPrPSc is able to seed recPrP or PrPC in RT-QuIC and PMCA reactions [19,29], infect susceptible cell lines to establish a chronically infected state [94], and cause bona fide prion disease in wild-type mice via an intracerebral, intraperitoneal, or oral route of infection [19,24,25].