Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL) studies of HCC tumor tissue sections confirmed that Ad-sp-VGLL4 promoted apoptosis in HCC in vivo, and HE-staining demonstrated that Ad-sp-VGLL4 inhibited angiogenesis and caused damage in HCC. This evidence concerns the gene VGLL4 and hepatocellular carcinoma.