CD4 and neoplasm: Strong correlations were seen between frequencies of FoxP3+Helios+ Tregs and CD4+CTLA-4+ T cells in PBMCs in patients with advanced tumor stages (r = 0.162, p = 0.594 [early]; r = 0.550, p = 0.018 [advanced]), while such correlation was observed in the TME of patients with early tumor stage (r = 0.911, p = 0.0006 [early]; r = 0.486, p = 0.129 [advanced]) (Figure 2D).