Strong correlations were found between frequencies of FoxP3+Helios− Tregs with CD4+LAG-3+ T cells and CD4+CTLA-4+ T cells in TILs in patients with early tumor stages, compared to advanced stages (r = 0.800, p = 0.013 [early]; r = 0.214, p = 0.478 [advanced]; r = 0.824, p = 0.006 [early]; r = 0.529, p = 0.093 [advanced], respectively) (Figure 3C,D). The gene discussed is CTLA4; the disease is neoplasm.