Consistent with these data, we found strong correlations between frequencies of CD4+CTLA-4+ T cells and FoxP3+ Tregs, FoxP3+Helios+ Tregs, and FoxP3−Helios− T cells in the TME of CRC patients with early stages, indicating the beneficial immunosuppressive role of CD4+CTLA-4+ T cells in the TME of CRC patients. This evidence concerns the gene CD4 and colorectal carcinoma.