In summary, our results reveal that GLE prevents MPTP-induced neuroinflammation, and an in vitro study thus provides the implication that anti-inflammatory mechanism may be partially involved in suppression of NLRP3 activation and microglial autophagy deficiency, suggesting that Ganoderma lucidum could be chosen for targeting microglia as a therapeutic intervention in PD. This evidence concerns the gene NLRP3 and Parkinson disease.