Afterward, the expression levels of fibrosis-related genes in both groups of mice were explored, resulting in the discovery that the mRNA levels of α-SMA, COL1A1, and COL3A1 were reduced in WD-fed mice receiving HXT + VitE treatment compared to WD-fed mice receiving the vehicle alone (Figure 4C–E). The gene discussed is COL3A1; the disease is Wilson disease.