In the current study, the results showed that Asp treatment decreased the apoptosis rate of colonic epithelial cells and the protein expression of MLKL in the colon of UC mice, while it increased the protein expression of RIPK1 and RIPK3, suggesting that the rectal infusion of different levels of Asp could effectively inhibit cell apoptosis and necrosis caused by colitis. This evidence concerns the gene MLKL and colitis.