Figure 3 shows the plasma levels of S100A9, AACT, AFM, TAOK3, NGAL, PSMA3, and AMBP proteins. The results show that the CCA patients had significantly higher plasma S100A9, AACT, NGAL, and PSMA3 levels relative to the normal controls (Figure 3). The plasma S100A9 and AACT proteins of the CCA group were also significantly higher than those of the DC group (Figure 3). The gene discussed is SERPINA3; the disease is cholangiocarcinoma.