In a similar study, treatment of mammary gland with 25(OH)D3 in cattle with LPS induced mastitis showed total milk somatic cells having upregulated expression of NOS2, RANTES/CCL5, DEFB3, DEFB4, DEFB7, DEFB10, IL1B, and IL8, with most effects being observed between 4–8 h following treatment [209]. This evidence concerns the gene NOS2 and mastitis.