Based on our previous results that suggested the genetic effects of phospholipase-containing domain 3 (PNPLA3) rs738409, transmembrane 6 superfamily member 2 (TM6SF2) rs58542926, and the sorting and assembly machinery component 50 homolog (SAMM50) rs2073080 and rs3761472 on the development and severity of pediatric NAFLD were greater in the overweight group than the normal-weight group [13], we sought to find metabolic signatures associated with pediatric NAFLD in a subpopulation of the genetics study. Here, PNPLA3 is linked to metabolic dysfunction-associated steatotic liver disease.