We have induced insulin resistance using a staged approach of culture for a week in maturation media containing 5 mM glucose supplemented with 0.4 mM oleic acid, followed by glucose-free insulin resistance media containing 0.3 mM palmitic acid and 50 nM insulin, and then a further three days with 12 mM glucose added to the insulin resistance media [111]. This evidence concerns the gene INS and Insulin resistance.