TNF and bone neoplasm: Thus, RT-qPCR was performed to detect the expression of inflammatory factors and the results showed that the expressions of Tnf, Il1b, and Il6 in the BCP group were significantly higher than those in the sham-operated group (unpaired t-test of Tnf: p = 0.0238, BCP vs. sham-operated group; unpaired t-test of Il1b: p = 0.0009, BCP vs. sham-operated group; unpaired t-test of Il6: p = 0.0287, BCP vs. sham-operated group; Figure 3C–E), indicating the involvement of neuroinflammation in the development of bone cancer pain.