Because WGP might have a different influence on different subtypes of macrophages, WGP was incubated in several macrophage manipulation protocols, including M0 (control), M1 (activation by lipopolysaccharide; LPS), M2 (IL-4 stimulation), and TAM (tumor supernatant incubation), to explore tumoricidal activity (Figure 4A). The gene discussed is IL4; the disease is neoplasm.