A previous study by our group characterized, for the first time, the protein composition of EVs secreted by pathological murine C3H10T1/2 adipocytes with insulin resistance and/or lipid hypertrophy, by combining treatments with high-dose glucose and insulin or with fatty acids; thus, observing different protein charges, and therefore, possible biomarkers depending on the cell stimulus of origin [7]. This evidence concerns the gene INS and Insulin resistance.