In turn, EVs secreted by BAT from obese animals presented a higher ratio of upregulated proteins related to signal transduction, cell communication, inflammation, and obesity, such as annexin A6, MIF (macrophage migration inhibitory factor), CD14 (monocyte differentiation antigen), and ceruloplasmin (Figure 5C,D and Figure 6A, Supplementary Materials File S2). This evidence concerns the gene ANXA6 and obesity due to melanocortin 4 receptor deficiency.