The heatmap of the top 3 specific TFs of each cell type showed the activation status of specific regulatory factors in each cell type, among which only 10 TFs (Bhlhe22, Lhx9, Ovol2, Cux2, Uncx, Rarb, Sox9, Emx2, Tbx2, Nr1h3) were co-activated in the same cell types of PD and CN, but the regulatory intensity was different (Figure 2B). The gene discussed is BHLHE22; the disease is Parkinson disease.