Based on our reported observations showing lower levels of intact Aβ40/42 [18] and AChE [19] and higher levels of mBDNF [24] in H1299 cell media, we hypothesized that sAPPα levels are regulated by AChE and mBDNF in the media of A549 and H1299 human NSCLC cell lines and identified signaling pathways involved in this regulation. The gene discussed is ACHE; the disease is non-small cell lung carcinoma.