BAG3 and ischemia: Similar to the mRNA results, BAG3 protein expression was also significantly increased at 3 h after exposure to simulated ischemia and remained elevated during the experiment when compared to cells grown under non-ischemic conditions (Figure 1B,C; non-ischemic, 1.0 ± 0.13; 3 h simulated ischemia, 2.71 ± 0.55; 6 h simulated ischemia, 1.95 ± 0.07; 12 h simulated ischemia, 1.94 ± 0.05, * p < 0.05).