Exposure of BAG3-depleted HSMCs to 6 h of simulated ischemia was associated with decreased LC3-II/-I ratio, suggesting decreased autophagy (Figure 3C,E; LC3-II/-I ratio in shControl vs. shBAG3, 0.45 ± 0.03 vs. 0.30 ± 0.01, * p < 0.05). Here, BAG3 is linked to ischemia.