Lower BAG3 expression was associated with lower expression of autophagy markers ATG5 (Figure 4A,C; NCD vs. HFD, 1.0 ± 0.03 vs. 0.06 ± 0.02, * p < 0.05), ATG7 (Figure 4A,D; NCD vs. HFD, 0.99 ± 0.17 vs. 0.35 ± 0.03, * p < 0.05), and undetectable band intensity of LC3-II (Figure 4A,E; NCD vs. HFD, 0.04 ± 0.004 vs. not detected) in ischemic GA muscle of mice with diabetes compared to non-diabetic controls. The gene discussed is ATG5; the disease is diabetes mellitus.