To validate the ACTC1 and NHLRC1 lung tumor DMRs and to confirm their differential expression in lung tumors, we performed sequence-specific methylation-sensitive mass spectrometry (MassARRAY) and real-time PCR (rtPCR) in 94 lung squamous cell carcinoma (SCC) and in lung adenocarcinoma (ADC) versus paired adjacent normal lung tissue samples from current or former smokers of the lung tumor versus adjacent normal sample set (Table 3). The gene discussed is ACTC1; the disease is squamous cell lung carcinoma.