Detailed analysis of the ACTC1 region showed that three CpGs (cg10580056, cg03844894 and cg05432213) adjacent to cg17242351 were hypermethylated in lung cancer versus normal tissue and in addition, ACTC1 expression was reduced in lung tumor tissue from TCGA (Supplementary Figure S1B–E). This evidence concerns the gene ACTC1 and lung cancer.