AKT1 and familial pancreatic carcinoma: Even though we were not able to confirm those data at the protein level using different approaches such as Western blot, enzyme-linked immunosorbent assay or immunofluorescence, stimulation of pancreatic cancer cell lines with exogenous apelin induced the phosphorylation of extracellular signal-regulated kinases (ERKs), as well as Akt, revealing APJ expression (Figure A5b).